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Chemosystematics in the Opiliones (Arachnida): a comment on the evolutionary history of alkylphenols and benzoquinones in the scent gland secretions of Laniatores 下载免费PDF全文
Günther Raspotnig Michaela Bodner Sylvia Schffer Stephan Koblmüller Axel Schnhofer Ivo Karaman 《Cladistics : the international journal of the Willi Hennig Society》2015,31(2):202-209
Large prosomal scent glands constitute a major synapomorphic character of the arachnid order Opiliones. These glands produce a variety of chemicals very specific to opilionid taxa of different taxonomic levels, and thus represent a model system to investigate the evolutionary traits in exocrine secretion chemistry across a phylogenetically old group of animals. The chemically best‐studied opilionid group is certainly Laniatores, and currently available chemical data allow first hypotheses linking the phylogeny of this group to the evolution of major chemical classes of secretion chemistry. Such hypotheses are essential to decide upon a best‐fitting explanation of the distribution of scent‐gland secretion compounds across extant laniatorean taxa, and hence represent a key toward a well‐founded opilionid chemosystematics. 相似文献
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Compartment‐specific aggregases direct distinct nuclear and cytoplasmic aggregate deposition 下载免费PDF全文
Annett Neuner Mohamed YH Mohamed D Lys Guilbride Karsten Richter Michael Lisby Elmar Schiebel Axel Mogk Bernd Bukau 《The EMBO journal》2015,34(6):778-797
Disruption of the functional protein balance in living cells activates protective quality control systems to repair damaged proteins or sequester potentially cytotoxic misfolded proteins into aggregates. The established model based on Saccharomyces cerevisiae indicates that aggregating proteins in the cytosol of eukaryotic cells partition between cytosolic juxtanuclear (JUNQ) and peripheral deposits. Substrate ubiquitination acts as the sorting principle determining JUNQ deposition and subsequent degradation. Here, we show that JUNQ unexpectedly resides inside the nucleus, defining a new intranuclear quality control compartment, INQ, for the deposition of both nuclear and cytosolic misfolded proteins, irrespective of ubiquitination. Deposition of misfolded cytosolic proteins at INQ involves chaperone‐assisted nuclear import via nuclear pores. The compartment‐specific aggregases, Btn2 (nuclear) and Hsp42 (cytosolic), direct protein deposition to nuclear INQ and cytosolic (CytoQ) sites, respectively. Intriguingly, Btn2 is transiently induced by both protein folding stress and DNA replication stress, with DNA surveillance proteins accumulating at INQ. Our data therefore reveal a bipartite, inter‐compartmental protein quality control system linked to DNA surveillance via INQ and Btn2. 相似文献
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Captive breeding has become an important tool in species conservation programmes. Current management strategies for ex situ populations are based on theoretical models, which have mainly been tested in model species or assessed using studbook data.
During recent years an increasing number of molecular genetic studies have been published on captive populations of several
endangered species. However, a comprehensive analysis of these studies is still outstanding. Here, we present a review of
the published literature on ex situ conservation genetics with a focus on molecular studies. We analysed 188 publications which either presented empirical studies
using molecular markers (105), studbook analyses (26), theoretical work (38), or tested the genetic effects of management
strategies using model species (19). The results show that inbreeding can be minimized by a thorough management of captive
populations. There seems to be a minimum number of founders (15) and a minimum size of a captive population (100) necessary
in order to minimize a loss of genetic diversity. Optimally, the founders should be unrelated and new founders should be integrated
into the captive population successively. We recommend that genetic analyses should generally precede and accompany ex situ conservation projects in order to avoid inbreeding and outbreeding depression. Furthermore, many of the published studies
do not provide all the relevant parameters (founder size, captive population size, Ho, He, inbreeding coefficients). We, therefore, propose that a general standard for the presentation of genetic studies should
be established, which would allow integration of the data into a global database. 相似文献
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Maren Ilowski Axel Kleespies Enrico N. de Toni Barbara Donabauer Karl-Walter Jauch Jan G. Hengstler Wolfgang E. Thasler 《Biochemical and biophysical research communications》2011,(1):148
Augmenter of liver regeneration (ALR) is known to support liver regeneration and to stimulate proliferation of hepatocytes. However, it is not known if ALR exerts anti-apoptotic effects in human hepatocytes and whether this protective effect is cell type specific. This is relevant, because compounds that protect the liver against apoptosis without undesired effects, such as protection of metastatic tumour cells, would be appreciated in several clinical settings. Primary human hepatocytes (phH) and organotypic cancer cell lines were exposed to different concentrations of apoptosis inducers (ethanol, TRAIL, anti-Apo, TGF-β, actinomycin D) and cultured with or without recombinant human ALR (rhALR). Apoptosis was evaluated by the release of cytochrome c from mitochondria and by FACS with propidium iodide (PI) staining.ALR significantly decreased apoptosis induced by ethanol, TRAIL, anti-Apo, TGF-β and actinomycin D. Further, the anti-apoptotic effect of ALR was observed in primary human hepatocytes and in HepG2 cells but not in bronchial (BC1), colonic (SW480), gastric (GC1) and pancreatic (L3.6PL) cell lines.Therefore, the hepatotrophic growth factor ALR acts in a liver specific manner with regards to both its mitogenic and its anti-apoptotic effect. Unlike the growth factors HGF and EGF, rhALR acts in a liver specific manner. Therefore, ALR is a promising candidate for further evaluation as a possible hepatoprotective factor in clinical settings. 相似文献
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